The Ueda Group

Department of Chemistry & Biotechnology, Faculty of Engineering, The University of Tokyo
Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo

Takuya Ueda

Recent Publications

  • PNAS 2016, 113, E1180–E1189. [PDF]
  • Molecular Cell 2015, 59, 125–132. [PDF]
  • Nature Protocols 2015, 10, 1328–1344. [PDF]
  • Angew. Chem. Int. Ed. 2014, 53, 7535–7538. [PDF]


Protein synthesis is the most fundamental process in life. We address elucidation of a basic principle of translation systems derived various living organisms, such as bacteria, eukaryote and mitochondria. We are challenging creation of artificial cell based on cell-free translation system.

Biochemistry of translation system :

Using the cell-free translation systems (PURE systems) reconstituted with purified translation factors originated of E. coli, yeast and mitochondria, we are promoting biochemical studies to elucidate mechanism of translation process.

Single molecule imaging of translation system :

Our research aims to visualize dynamic actions of translation process using single-molecule observation techniques.

Synthetic Biology based on the PURE system :

By using the PURE system, we are challenging the syntheses of super molecular complexes such as ribosome and membrane protein complexes such as ATPase. The goal of the project is creation of artificial cell.

Development of medical probes using cell-free evolution systems :

We are searching protein probes including antibodies using ribosome-display based on the PURE system to develop drugs for diagnosis and therapy.