Protein Engineering Group

Department of Chemistry & Biotechnology
Faculty of Engineering / School of Engineering, The University of Tokyo

http://park.itc.u-tokyo.ac.jp/hueda/

  Associate Professor
Hiroshi Ueda

Recent Publications

  • J. Am. Chem. Soc. 2011, 133, 17386-17394. [PDF]
  • Bioconj. Chem. 2011, 22, 633-641. [PDF]
  • Anal. Chem. 2011, 83, 1008-1014. [PDF]
  • Lab. Chip 2010, 10, 92-100. [PDF]

Research

We aim at the creation of novel functional proteins (molecular machines) and novel methods based on them. To this end, combination of rational design and library selection are employed to surpass natural useful proteins with high recognition and/or catalytic activity.

Development of novel immunoassay by antibody engineering approach :

The utility of our novel noncompetitive 'Open-Sandwich' immunoassay based on the principle of antigen-dependent stabilization of antibody variable region, which is proven suitable for sensitive detection of many small molecules, is investigated. In addition, its integration with various nanotechnologies is attempted to further improve its utility.

Development of various biosensor proteins :

Our research aims to create novel useful functional proteins those include sensor proteins for specific DNA, or allosteric enzyme that can recognize any molecule as an effector.

Reaction mechanism of bioluminescent enzymes and its application :

Based on the analysis of firefly luciferase reaction mechanism, a rapid and sensitive protein-protein interaction assay is being developed.